8:50 am Chair’s Opening Remarks
8:55 am
Enhancing the Translational Confidence of T Cell–Redirection Strategies
Synopsis
Multiple methodologies exist for directing T cells to tumor cells. Checkpoint, CAR and BITE’s have generated much excitement given the range and level of function they can elicit from T-cells as well as the expansion of treatment possibilities they present for immunotherapies. This session will look to review emerging preclinical strategies for predicting, mediating and mitigating toxicities.
9:00 am Validating IO Bispecific Antibodies in Humanized Models
Synopsis
• Animal models for pre-clinical efficacy evaluation for ABL Bio’s bispecific antibodies
• Sharing model validation approaches
• Humanized PBMC models, Humanized CD34+ HSC models
9:30 am Preclinical Development of T Cell Redirecting Bispecific Antibodies
Synopsis
• Get an overview of the key challenges faced when carrying out preclinical development of bispecific antibodies
• Learn how the PDX/Syngeneic models available can be used effectively to overcome these challenges
• How to evaluate T cell induced cytokine storms in preclinical models
10:00 am Morning Refreshments & Networking
11:00 am IL12-Armored CAR-T: Multi-Dimensional Data Analysis to Assess both Efficacy & Safety Considerations in an NSG Mouse Model
Synopsis
• 4th generation “Armored” CAR-T cells express a biologic payload to assist in overcoming hostile tumor microenvironments
• Many payloads, such as IL12, have potentially toxic effects when dosed systemically
• Chimera’s proprietary GOLD CAR-T cells restrict the payload delivery to the local tumor microenvironment
• We used measurements of IL12 levels and other pro-inflammatory cytokines in the blood correlated with tumor burden and time-since-tumor-clearance to compare inducible verses constitutive IL12 delivery by CAR-T cells
• Combining a temporal analysis with quantitation of both payload and effector cytokines gives a more complete picture of the benefit of GOLD-controlled delivery of IL-12 verses constitutive delivery
3D Oncology Modeling & its Applications in IO
Synopsis
3D oncology modeling has seen a resurgence in recent times due to the versatility and cost-effectiveness they provide across a variety of cancers. This session will see speakers highlight the new capabilities that 3D models offer preclinical teams.
11:30 am The Potential of 3D Tumor Organoid Models in Testing ImmunoOncology Therapeutics
Synopsis
• Get an overview on the 3-D hydrogel system, particularly synthetic/ polymeric; PDX use in vitro; and microfluidic perfusion plates
• Discussing the incorporation of more immune components and IO aspects in our current model
12:00 pm Lunch & Networking
1:00 pm Modeling Immune Mediated Beta Cell Destruction in Human Type 1 Diabetes With Islet Organoids
Synopsis
• Organ-immune interactions are critical and often under-appreciated and we can derive important learnings from human organoid models for autoimmune diseases, some of which might be rather helpful for the tumor immunology field
• Islet beta cell stress is a critical component of immune destruction
• We have created, an islet organoid model that allows for precise evaluation of islet stress, also via imaging, and immune attacks. The key here is the reliability and small deviations of the system, which is assured by the microfluidic device and using organoids
1:30 pm Panel Discussion: Recap – 3D Oncology Modeling
Synopsis
• 3D modeling and its capabilities have been somewhat of a blind spot for preclinical and translational oncologists. In this discussion we will lift the lid on these models to reveal what the future may hold for research teams that are looking to embrace these technologies
• As well as the capabilities, we will also consider the short comings and the areas that are yet to be explored
2:00 pm Afternoon Refreshments & Networking
Modeling Strategies to Enhance the Translational Confidence of Immunotherapy Combinations
Synopsis
The renaissance in cancer immunotherapy is bringing with it added complexity for combinatorial drug development. Given the lack of predictiveness often attributed to current models, huge question marks still center around key study design elements such as dosing, scheduling, escalation strategies and patient
selection. This section will look to elucidate the understanding of the mechanism of action of tested
compounds and help with identifying rationale combination partners for best anti-tumor efficacy.
3:00 pm Supercharging the Tumor Microenvironment with the Engineered Cytokines NKTR-214 & KNTR-255
Synopsis
• The idea of combining the immune modulating properties of checkpoint inhibitors and other immunological medicines with the immune stimulating function of engineered cytokines is conceptually powerful
• Engineered cytokines can more effectively stimulate cytokine receptor pathways, while controlling adverse events
• The combination of NKTR-214 with Opdivo has demonstrated powerful anti-tumor effects and profoundly alters the tumor microenvironment, increasing effector T-cell counts, increasing PD-1 expression on tumor T-cells, and converting PD-L1 negative tumors to positive, while maintaining a more tolerable AE profile than traditional cytokine therapies
• NKTR-255, an IL-15 receptor agonist stimulates NK cells and CD8 memory T cells has the ability to be combined with monoclonal antibodies with ADCC function or with cellular therapies
3:30 pm Engage, Expand, Enable: Therapy of Established Tumors with Multiple Agents Targeting Diverse Immune-Tumor Interactions
Synopsis
• Animal model selection and therapeutic success is crucial to moving into clinical trials
• Optimal tumor therapy requires addressing multiple tumor-immune cell interactions